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Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals
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Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

DOI:
10.3791/59442-v

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08:22 min

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February 20, 2020

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, , , , ,
1Department of Veterinary Pathobiology,University of Missouri, 2College of Veterinary Medicine,University of Missouri, 3Department of Animal Science,University of Missouri, 4School of Natural Resources,University of Missouri, 5USDA USFS Northern Research Station

Capítulos

  • 00:05Introduction
  • 01:07Sterile, Isotonic, Salinated Deuterium Oxide (D2O) Preparation
  • 02:38Big Brown Bat (Eptesicus fucsus) Body Composition Determination
  • 04:34Fourier-transform Infrared (FTIR) Spectrophotometry Analysis
  • 06:20Results: Representative D2O Body Composition Assessment Validation
  • 07:38Conclusion

Summary

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This article describes the deuterium oxide dilution technique in two mammals, an insectivore and carnivore, to determine total body water, lean body mass, body fat mass, and water consumption.

Tags

Keywords: Deuterium OxideD2ONon-invasiveNon-lethalBody CompositionWater ConsumptionMammalsStable IsotopeWaterEndangered SpeciesHumansDomestic SpeciesDeuterium Oxide DilutionFree-ranging WildlifeRecapture RatesSocially Housed AnimalsAnesthesiaSubcutaneous InjectionsStock SolutionSodium ChlorideSterileVacuumIsosmotic StrengthInterfemoral VeinPetroleum Jelly29-gauge Needle

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