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Journal / Biochemistry / 通过对Rho激酶活性的暂时抑制,增强人类诱导多能干细胞衍生心肌细胞的诱导…
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Biochemistry

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JoVE Journal Biochemistry
通过对Rho激酶活性的暂时抑制,增强人类诱导多能干细胞衍生心肌细胞的诱导
 
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通过对Rho激酶活性的暂时抑制,增强人类诱导多能干细胞衍生心肌细胞的诱导

Article DOI: 10.3791/59452-v • 08:00 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1,2, 1, 1,3, 1, 1, 1, 2, 1, 1
1Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering, University of Alabama at Birmingham, 3Department of Cardiac Surgery, The Second Xiangya Hospital of Central South University

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在该协议中,我们演示并阐述了如何使用人类诱导多能干细胞进行心肌细胞分化和纯化,并进一步说明如何利用Rho相关蛋白激酶抑制剂提高移植效率小鼠心肌梗死模型的预处理。

Tags

生物化学,第149期,多能干细胞,心肌细胞,细胞治疗,心肌梗死,红激酶,ROCK抑制剂
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