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Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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JoVE Journal Pesquisa do Câncer
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

DOI:
10.3791/59460-v

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11:44 min

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March 30, 2019

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, , , , , , ,
1School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy,University of Louisiana Monroe, 2Department of Pharmaceutical Sciences, School of Pharmacy,Texas Tech University Health Sciences Center, 3Department of Obstetrics and Gynecology, School of Medicine,Texas Tech University Health Sciences Center

Capítulos

  • 00:00Título
  • 01:08Transfection
  • 03:08RNA Extraction
  • 04:53cDNA Synthesis and qPCR
  • 06:28Cell Cycle Analysis
  • 08:13Antibody Protein Microarray
  • 09:34Representative Results
  • 11:09Conclusion

Summary

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Tadução automática

miRNA therapeutics have significant potential in regulating cancer progression. Demonstrated here are analytical approaches used for identification of the activity of a combinatorial miRNA treatment in halting cell cycle and angiogenesis.

Tags

Lung CancerMicroRNACell CycleAngiogenesisGene ExpressionRNA ExtractionQPCRMicroarrayTransfectionCell CultureCell HarvestingRNA Isolation

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