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Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode

Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode

DOI:
10.3791/59487-v

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07:10 min

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March 27, 2019

•

, , ,
1School of Biosciences,University of Melbourne, 2Melbourne School of Psychological Sciences,University of Melbourne, 3Department of Optometry and Vision Sciences,University of Melbourne

Capítulos

  • 00:04Título
  • 00:57Electrode Preparation
  • 02:44Zebrafish and Sponge Platform Preparation
  • 03:48Animal and Electrode Positioning
  • 05:38Results: Representative Electroretinogram (ERG) A- and B-Wave Amplitude Tracings
  • 06:37Conclusion

Summary

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Tadução automática

Here, we present a protocol that simplifies the measurement of light evoked electroretinogram responses from larval zebrafish. A novel cone-shaped sponge-tip electrode can help to make the study of visual development in larval zebrafish using the electroretinogram ERG easier to achieve with reliable outcomes and lower cost.

Tags

Keywords: ElectroretinogramERGLarval ZebrafishCone-shaped Sponge-tip ElectrodeVisual DevelopmentGenetic Disease ModelsElectrode ManufactureDark AdaptationRinger’s BufferPhotovoltaic Artifacts

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