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一种新型锥形海绵尖电极在幼虫斑马鱼中的电录记录

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode

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一种新型锥形海绵尖电极在幼虫斑马鱼中的电录记录

DOI:

10.3791/59487-v

•

07:10 min

•

March 27, 2019

•

Jiaheng Xie, Patricia R. Jusuf, Patrick T. Goodbourn, Bang V. Bui

¹School of Biosciences,University of Melbourne, ²Melbourne School of Psychological Sciences,University of Melbourne, ³Department of Optometry and Vision Sciences,University of Melbourne

Capítulos

00:04Título
00:57Electrode Preparation
02:44Zebrafish and Sponge Platform Preparation
03:48Animal and Electrode Positioning
05:38Results: Representative Electroretinogram (ERG) A- and B-Wave Amplitude Tracings
06:37Conclusion

Summary

Tadução automática

在这里, 我们提出了一个协议, 简化测量光诱发视网膜图像反应从幼虫斑马鱼。一种新型锥形海绵尖电极可以使利用电视图 erg 更容易地实现幼虫斑马鱼的视觉发育, 结果可靠, 成本更低。

Tags

Keywords: Electroretinogram ERG Larval Zebrafish Cone-shaped Sponge-tip Electrode Visual Development Genetic Disease Models Electrode Manufacture Dark Adaptation Ringer’s Buffer Photovoltaic Artifacts

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