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1Oxford Centre for Diabetes, Endocrinology and Metabolism,University of Oxford, 2Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre,Malmö University HospitalMalmö, 3Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit,University of Göteborg, 4Oxford National Institute for Health Research,Biomedical Research Centre, 5Life and Medical Sciences,University of Hertfordshire
Capítulos
- 00:04Título
- 00:30Calcium (Ca2+) Imaging Dynamics
- 02:21Microscope Setup
- 03:48Time-Lapse Imaging
- 04:45Numerical Data Analysis
- 06:16Results: Representative Islet Cell Responses to Various Stimuli
- 07:26Conclusion
Her præsenterer vi en protokol for billeddannelse og kvantificering af calcium dynamik i heterogene cellepopulationer, såsom pancreas-ø-celler. Fluorescerende reportere leveres ind i det perifere lag af celler inden for øen, som derefter er immobiliseret og imaged, og per-celle analyse af dynamikken i fluorescens intensitet udføres.
