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Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture
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Biologia
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JoVE Journal Biologia
Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

DOI:
10.3791/59505-v

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15:31 min

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October 23, 2019

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, , , , , , ,
1Department of Geology & Geophysics,Yale University, 2Department of Ecology & Evolution,Stony Brook University, 3Neurogenetics of Vocal Communication,Max Planck Institute for Psycholinguistics, 4School of Biological and Chemical Sciences,Queen Mary University of London, 5School of Biology & Environmental Science,University College Dublin, 6Donders Institute for Brain, Cognition and Behavior, 7Consortium for Inter-Disciplinary Environmental Research,Stony Brook University

Capítulos

  • 00:04Título
  • 00:52Cranial Dissections for RNA
  • 04:05Postcranial Dissections for RNA
  • 06:24Tissue Culture
  • 09:41Refreshing Media and Passaging Cells
  • 11:38Conclusion
  • 13:15Freezing Viable Living Cells and Thawing the Frozen Cells
  • 14:48Results: Tissue Preparation and Cell Culture Results

Summary

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This is a protocol for the optimal tissue preparation for genomic, transcriptomic, and proteomic analyses of bats caught in the wild. It includes protocols for bat capture and dissection, tissue preservation, and cell culturing of bat tissue.

Tags

-omics AnalysesPrimary Cell CultureHumane SamplingRNA PreservationBrain DissectionOlfactory BulbCochleaSpecimen Identification

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