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Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments
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JoVE Journal Medicina
Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments

DOI:
10.3791/59514-v

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06:08 min

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May 26, 2019

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, , ,
1Department of Cancer Biology,Cleveland Clinic, 2Department of Radiation Oncology,Cleveland Clinic

Capítulos

  • 00:04Título
  • 01:05Cell Preparation for Suspension Cell Culture
  • 02:53Cell Preparation for Attached Cell Culture
  • 03:40Irradiation with a Linear Accelerator (LINAC)
  • 04:52Results: Cell Cycle Effect of Standard Dose Rate and Extra High Dose Rate Irradiation by a Linear Accelerator
  • 05:23Conclusion

Summary

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Tadução automática

Clinical linear accelerators can be used to determine biologic effects of a wide range of dose rates on cancer cells. We discuss how to set up a linear accelerator for cell-based assays and assays for cancer stem-like cells grown as tumorspheres in suspension and cell lines grown as adherent cultures.

Tags

Linear AcceleratorRadiation TherapyRadiobiologyIn Vitro ExperimentsGlioma Stem-like CellsCell CultureCell IrradiationDosimetryDosimetric CalculationsMonitor Units

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