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Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

DOI:
10.3791/59517-v

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09:04 min

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May 28, 2019

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, ,
1Department of Microbiology and Immunology,University of North Carolina at Chapel Hill, 2Department of Biology,University of North Carolina at Chapel Hill

Capítulos

  • 00:04Título
  • 00:24Preparation of Arabidopsis thaliana Seedlings on a Plastic Mesh
  • 01:36Colonization of Plants in Liquid Bacterial Growth Medium
  • 03:16Bacterial Colonization Maintenance
  • 04:14Collection of Bacteria for Viable Cell Counts
  • 05:19Collection of Intact Plant Roots for Microscopy
  • 06:13Results: Assessment of Bacterial Colonization on Arabidopsis thaliana Roots
  • 08:39Conclusion

Summary

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Here we describe a hydroponic plant growth assay to quantify species presence and visualize the spatial distribution of bacteria during initial colonization of plant roots and after their transfer into different growth environments.

Tags

Keywords: Bacterial ColonizationArabidopsis ThalianaRootsFloating Hydroponic SystemPlant-microbe InteractionsMesh DisksSeed GerminationBacterial Growth MediumColony-forming UnitsGas-permeable FilmPlant Growth Chamber

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