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在浮动水培系统中监测阿拉伯菌的殖民化与维护

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

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在浮动水培系统中监测阿拉伯菌的殖民化与维护

DOI:

10.3791/59517-v

•

09:04 min

•

May 28, 2019

•

Susanna L. Harris, Cesar A. Pelaez, Elizabeth A. Shank²

¹Department of Microbiology and Immunology,University of North Carolina at Chapel Hill, ²Department of Biology,University of North Carolina at Chapel Hill

Capítulos

00:04Título
00:24Preparation of Arabidopsis thaliana Seedlings on a Plastic Mesh
01:36Colonization of Plants in Liquid Bacterial Growth Medium
03:16Bacterial Colonization Maintenance
04:14Collection of Bacteria for Viable Cell Counts
05:19Collection of Intact Plant Roots for Microscopy
06:13Results: Assessment of Bacterial Colonization on Arabidopsis thaliana Roots
08:39Conclusion

Summary

Tadução automática

在这里,我们描述了一种水培植物生长测定,用于量化物种存在,并可视化细菌在植物根系初始殖民化期间和转移到不同生长环境后的空间分布。

Tags

Bacterial Colonization Arabidopsis Thaliana Roots Floating Hydroponic System Plant-microbe Interactions Mesh Disks Seed Germination Bacterial Growth Medium Colony-forming Units Gas-permeable Film Plant Growth Chamber

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