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Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity
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Biologia
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JoVE Journal Biologia
Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

DOI:
10.3791/59528-v

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07:16 min

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May 06, 2019

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, ,
1Department of Physiology and Biophysics,University of California, Irvine, 2Department of Developmental and Cell Biology,University of California, Irvine

Capítulos

  • 00:04Título
  • 01:08Larval and Adult Zebrafish Lens Dissection
  • 02:50Anterior-posterior Axis Lens Nucleus Localization Measurement
  • 04:18Distance from the Center of the Lens Nucleus to the Anterior Pole Measurement (a – r)
  • 05:10Results: Representative In Vitro and In Vivo Larval Zebrafish Lens Morphology Comparison
  • 06:36Conclusion

Summary

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These protocols were developed to analyze cortical lens morphology, structural integrity of the zebrafish lens sutures in fixed and live lenses and to measure the position of the zebrafish lens nucleus along the anterior-posterior axis.

Tags

Keywords: ZebrafishLensNucleus LocalizationSutural IntegrityLens DevelopmentLens PhysiologyReverse Genetic ScreeningLens Membrane MorphologyLens OpticsCataractLens HarvestLens ExcisionLens DissectionAnterior-posterior AxisLens Nucleus

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