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Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid
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JoVE Journal Medicina
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid

Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid

DOI:
10.3791/59532-v

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10:42 min

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April 11, 2019

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, ,
1Division of Hematology/Oncology, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai

Capítulos

  • 00:04Título
  • 01:12Buffer and Media Preparation
  • 01:52Isolation of Mononuclear Cells (MNCs) from Umbilical Cord Blood (UCB) Unit
  • 03:59Isolation of CD34+ Cells from UCBs
  • 06:29VPA Ex Vivo Expansion of Purified UCB-CD34+ Cells
  • 07:18Results: Analysis of the Ex Vivo Expansion of Hematopoietic Stem Cells
  • 09:30Conclusion

Summary

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Here, we describe the ex vivo expansion of hematopoietic stem cells from CD34+ cells derived from umbilical cord blood treated with a combination of a cytokine cocktail and VPA. This method leads to a significant degree of ex vivo expansion of primitive HSCs for either clinical or laboratory applications.

Tags

Ex Vivo ExpansionHematopoietic Stem CellsUmbilical Cord BloodCD34+ CellsValproic AcidRegenerative MedicineTransplantation TherapyCytokine CocktailGene EditingCell SeparationMononuclear CellsDensity GradientPurification

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