Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

Leighton  H. Duncan; Mark W. Moyle; Lin Shao; Titas Sengupta; Richard Ikegami; Abhishek Kumar; Min Guo; Ryan Christensen; Anthony Santella; Zhirong Bao; Hari Shroff; William Mohler; Daniel  A. Col&#243;n-Ramos

doi:10.3791/59533

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Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

DOI:

10.3791/59533-v

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08:16 min

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June 06, 2019

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Leighton H. Duncan⁵, Mark W. Moyle⁵, Lin Shao⁵, Titas Sengupta⁵, Richard Ikegami⁵, Abhishek Kumar⁵, Min Guo⁵, Ryan Christensen⁵, Anthony Santella⁵, Zhirong Bao⁵, Hari Shroff⁵, William Mohler⁵, Daniel A. Colón-Ramos^5,6

¹Department of Neuroscience and Department of Cell Biology,Yale University School of Medicine, ²Developmental Biology Program,Sloan Kettering Institute, ³Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling,University of Connecticut Health Center, ⁴Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health, ⁵WormGUIDES.org, ⁶Instituto de Neurobiología, Recinto de Ciencias Médicas,Universidad de Puerto Rico

Capítulos

00:04Título
00:34C. elegans Embryo Sample Mounting Preparation
01:48Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Embryonic Imaging
03:12Isotropic High-Resolution Volumetric Data: Cell Lineage Analysis
06:06Results: Representative Cellular Identification and Single Cell Characterization of Neurite Outgrowth Dynamics
07:43Conclusion

Summary

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Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment.

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

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