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亚细胞分辨率的各向同性光片显微镜及细胞自动动力学分析
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
DOI:

08:16 min

June 06, 2019

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Capítulos

  • 00:04Título
  • 00:34C. elegans Embryo Sample Mounting Preparation
  • 01:48Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Embryonic Imaging
  • 03:12Isotropic High-Resolution Volumetric Data: Cell Lineage Analysis
  • 06:06Results: Representative Cellular Identification and Single Cell Characterization of Neurite Outgrowth Dynamics
  • 07:43Conclusion

Summary

Tadução automática

在这里, 我们提出了一个组合方法, 使用高分辨率显微镜, 计算工具, 和单细胞标记在活的线虫胚胎, 以了解单个细胞动力学在神经发育过程中。

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