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果蝇、果蝇细胞免疫应答的评估
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay
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果蝇、果蝇细胞免疫应答的评估

DOI:
10.3791/59543-v

•

05:50 min

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April 10, 2019

•

,
1Department of Biology,University of Maryland, 2Department of Cell Biology and Molecular Genetics,University of Maryland, 3Institute for Bioscience and Biotechnology Research,University of Maryland

Capítulos

  • 00:04Título
  • 00:30Fly Injection
  • 02:32Fly Imaging and Fluorescence Quantification and Normalization
  • 03:59Results: Representative In Vivo Phagocytosis Assay Analyses
  • 05:18Conclusion

Summary

Tadução automática

Tadução automática

该方案描述了一种在成虫体内吞噬试验的方法, 以量化吞噬细胞的识别和微生物感染的清除。

Tags

PhagocytosisDrosophila MelanogasterIn Vivo AssayImmune ResponseCellular ImmunityGenetic ScreenGenome-wide Association StudyPathogen RecognitionPathogen ClearanceTrypan BlueFluorescence MicroscopyImaging Analysis

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