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增殖人类抗原特异性CD4⁺ T细胞使用卡博曲莱辛苏奇尼米酯的定量

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Quantification of Proliferating Human Antigen-specific CD4⁺ T Cells using Carboxyfluorescein Succinimidyl Ester

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增殖人类抗原特异性CD4⁺ T细胞使用卡博曲莱辛苏奇尼米酯的定量

DOI:

10.3791/59545-v

•

07:00 min

•

June 04, 2019

•

Anthony R. Di Carluccio, Eleonora Tresoldi, Michelle So, Stuart I. Mannering²

¹Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, ²Department of Medicine,University of Melbourne, St. Vincent’s Hospital

Capítulos

00:04Título
00:41Whole Blood Peripheral Blood Mononuclear Cell (PBMC) Preparation
01:21CD4⁺ T Cell Preparation
03:16CD4⁺ T Cell Labeling
04:11Flow Cytometric Analysis
05:31Results: Representative CFSE-labeled, Activated CD4⁺ T Cell Proliferation
06:21Conclusion

Summary

Tadução automática

这里介绍的是一个协议,用于测量增殖CD4⁺ T细胞,以响应抗原蛋白或肽使用染料稀释。这种测定对稀有抗原特异性T细胞特别敏感,可以进行改性,以利于抗原特异性细胞的克隆。

Tags

Keywords: CD4+ T Cells Proliferation CFSE Antigen-specific Quantification Autoimmune Conditions Type 1 Diabetes PBMC Density Gradient Centrifugation Cell Culture Flow Cytometry

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