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用于细胞质 siRNA 传递的带电反应性聚合物纳米粒子的制备

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

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用于细胞质 siRNA 传递的带电反应性聚合物纳米粒子的制备

DOI:

10.3791/59549-v

•

09:09 min

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May 02, 2019

•

John Hendershot, Adam E. Smith, Thomas A. Werfel^2,3

¹Department of Chemical Engineering,University of Mississippi, ²Department of BioMolecular Sciences,University of Mississippi, ³Biomedical Engineering Program,University of Mississippi

Capítulos

00:04Título
00:49Preparation and Characterization of si-NPs (siRNA Nanoparticles)
02:00Physicochemical Characterization of si-NPs
05:04Determining in vitro Bioactivity of si-NPs
06:35Results: Comparison between si-NP 1 and si-NP 2 and siRNA Complexation
08:29Conclusion

Summary

Tadução automática

介绍了制备和表征含中子、ph 响应的 siRNA 纳米粒子的物理化学特性和生物活性的方法。讨论了成功的 siRNA 纳米胺的标准, 如大小、形态、表面电荷、siRNA 负载和基因沉默。

Tags

SiRNA Nanoparticles Cytosolic Delivery PH-responsive Neutrally-charged Dynamic Light Scattering Transmission Electron Microscopy Systemic Administration Bioavailability

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