Journal
/
Pesquisa do Câncer
/
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
JoVE Journal
Pesquisa do Câncer
This content is Free Access.
JoVE Journal Pesquisa do Câncer
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

DOI:
10.3791/59551-v

•

11:39 min

•

June 09, 2019

•

, , , , , ,
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory,National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center,National Cancer Center

Capítulos

  • 00:04Título
  • 01:18Cell Culture on Day 1
  • 04:37Preparation of Reagents and Pre-washing Centrifugal Filter Units
  • 06:17Cell Culture on Day 2
  • 07:24Extraction of Metabolites from Cultured Cells and Ultrafiltration of Cell Extracts
  • 08:55Results: Diamide Treatment
  • 10:42Conclusion

Summary

Tadução automática

Tadução automática

The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry.

Tags

Keyword Extraction:MetabolomicsCapillary Electrophoresis-mass SpectrometryCEMSAqueous MetabolitesCultured Adherent CellsCancer CellsMetabolite ExtractionBiomarkersChemotherapy ResponseCell CultureTrypsin-EDTACell SuspensionCentrifugation

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

Extraction of Extracellular Vesicles from Whole Tissue

An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells

Read Article