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毛细管电泳质谱法提取培养细胞中的水代谢物进行代谢组学分析

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Pesquisa do Câncer

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JoVE Journal Pesquisa do Câncer

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

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毛细管电泳质谱法提取培养细胞中的水代谢物进行代谢组学分析

DOI:

10.3791/59551-v

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11:39 min

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June 09, 2019

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Ami Maruyama², Kenjiro Kami, Kazunori Sasaki, Hajime Sato, Yuzo Sato², Katsuya Tsuchihara, Hideki Makinoshima^2,4

¹Shonai Regional Industry Promotion Center, ²Tsuruoka Metabolomics Laboratory,National Cancer Center, ³Human Metabolome Technologies, Inc, ⁴Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center,National Cancer Center

Capítulos

00:04Título
01:18Cell Culture on Day 1
04:37Preparation of Reagents and Pre-washing Centrifugal Filter Units
06:17Cell Culture on Day 2
07:24Extraction of Metabolites from Cultured Cells and Ultrafiltration of Cell Extracts
08:55Results: Diamide Treatment
10:42Conclusion

Summary

Tadução automática

本文的目的是描述从培养的粘附细胞中提取水代谢物的方案, 用于代谢组学分析, 特别是毛细管电泳质谱法。

Tags

Keyword Extraction: Metabolomics Capillary Electrophoresis-mass Spectrometry CEMS Aqueous Metabolites Cultured Adherent Cells Cancer Cells Metabolite Extraction Biomarkers Chemotherapy Response Cell Culture Trypsin-EDTA Cell Suspension Centrifugation

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