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Isolement et purification des périphytes cardiaques murines
JoVE Journal
Medicina
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JoVE Journal Medicina
Isolation and Purification of Murine Cardiac Pericytes
DOI:

10:49 min

August 16, 2019

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Capítulos

  • 00:04Título
  • 00:44Preparation of Animal, Procurement of Cardiac Tissue, and Dissociation of Heart Tissue
  • 03:28Preparation of the Crude Cell Mixture for Fluorescence Activating Cell Sorting (FACS)
  • 06:34Purification of Pericytes from Crude Cell Mixture Using FACS
  • 08:18Results: Analysis of Crude Cells and Characterization of Primary Isolated Cardiac Pericytes
  • 09:55Conclusion

Summary

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Nous avons optimisé un protocole pour isoler et purifier les péritéytes cardiaques murines pour la recherche fondamentale et l'étude de leur biologie et de leur potentiel thérapeutique.

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