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Isolamento e purificazione dei Periciti Cardiaci Murine
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Isolation and Purification of Murine Cardiac Pericytes
DOI:

10:49 min

August 16, 2019

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Capítulos

  • 00:04Título
  • 00:44Preparation of Animal, Procurement of Cardiac Tissue, and Dissociation of Heart Tissue
  • 03:28Preparation of the Crude Cell Mixture for Fluorescence Activating Cell Sorting (FACS)
  • 06:34Purification of Pericytes from Crude Cell Mixture Using FACS
  • 08:18Results: Analysis of Crude Cells and Characterization of Primary Isolated Cardiac Pericytes
  • 09:55Conclusion

Summary

Tadução automática

Abbiamo ottimizzato un protocollo per isolare e purificare i periciti cardiaci murini per la ricerca di base e lo studio del loro potenziale di biologia e terapia.

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