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Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
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Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

DOI:
10.3791/59593-v

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09:44 min

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September 05, 2019

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,
1Blood Cell Development and Function Program,Fox Chase Cancer Center

Capítulos

  • 00:05Título
  • 01:46Mitochondrial ROS Fluorogenic Dye Staining
  • 04:41Lineage Antibody Staining
  • 06:04Flow Cytometry Acquisition and Analysis
  • 07:24Results: Flow Cytometry Gating Strategies and Mitochondrial ROS Levels
  • 08:54Conclusion

Summary

Tadução automática

Tadução automática

We describe a method for using multiparameter flow cytometry to detect mitochondrial reactive oxygen species (ROS) in murine healthy hematopoietic stem and progenitor cells (HSPCs) and leukemia cells from a mouse model of acute myeloid leukemia (AML) driven by MLL-AF9.

Tags

Flow CytometryMitochondrial Reactive Oxygen SpeciesHematopoietic Stem CellsHematopoietic Progenitor CellsAcute Myeloid LeukemiaMLL-AF9Redox StateMetabolism

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