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Fremstilling af amyloid-β-udskilning af Alginat-Mikroperler til brug ved modellering af Alzheimers sygdom
JoVE Journal
Biologia
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JoVE Journal Biologia
Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer’s Disease

Fremstilling af amyloid-β-udskilning af Alginat-Mikroperler til brug ved modellering af Alzheimers sygdom

DOI:
10.3791/59597-v

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06:52 min

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July 06, 2019

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, , ,
1Division of Pharmacy and Optometry, School of Health Studies, Faculty of Biology, Medicine and Health,University of Manchester, 2Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health,University of Manchester

Capítulos

  • 00:04Título
  • 01:02Setting Up the Encapsulation System
  • 02:16Microbeads Fabrication
  • 04:10Testing and Use of Microbeads
  • 04:57Results: Testing of Amyloid–Secreting
  • 06:31Conclusion

Summary

Tadução automática

Tadução automática

Denne protokol illustrerer en celle indkapsling metode ved hurtig fysisk gelering af alginat at immobilisere celler. Opnåede mikroperler tillader kontrolleret og vedvarende sekretion af amyloid-β over tid og kan anvendes til at studere virkningerne af udskillet amyloid-β i in vitro-og in vivo-modeller.

Tags

Keywords: AmyloidAlginate MicrobeadsAlzheimer’s DiseaseCell EncapsulationControlled ReleaseBiomolecule ReleaseCell SuspensionAlginate SolutionEncapsulatorGelation Bath

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