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原子力显微镜罐体功能化,具有单T细胞或单粒子免疫单细胞力光谱

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

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原子力显微镜罐体功能化,具有单T细胞或单粒子免疫单细胞力光谱

DOI:

10.3791/59609-v

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10:06 min

•

July 10, 2019

•

Jiahuan Chen, Ying Xu, Yan Shi, Tie Xia

¹State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine,Peking Union Medical College, ²Institute for Immunology, School of Medicine,Tsinghua University

Capítulos

00:04Título
01:17AFM Cantilever Preparation
02:36Attaching Single T-cells to the Cantilever
04:58Force Spectroscopy of Single-pair T-cell/Dendritic Cell Interaction
05:34Cantilever Functionalization with Single Polystyrene Beads
07:44Results: Measuring Cell-Cell Adhesions
09:30Conclusion

Summary

Tadução automática

我们提出了一种协议,用单个T细胞和珠子粒子使原子力显微镜(AFM)的吊带功能化,用于免疫学研究。显示了通过AFM探测单对T细胞树突状细胞结合的过程,并监测宏噬细胞对单个固体颗粒的实时细胞响应,并采用荧光成像。

Tags

Atomic Force Microscope Cantilever Functionalization Single T Cell Single Particle Immunological Force Spectroscopy Dendritic Cell-T Cell Interaction Cell-surface Interaction Biocompatible Glue IL-2 Sample Preparation Calibration Living Cell Environment

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