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蛋白质的表征(按尺寸-排除色谱与多角度光散射相结合(SEC-MALS)
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
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蛋白质的表征(按尺寸-排除色谱与多角度光散射相结合(SEC-MALS)

DOI:
10.3791/59615-v

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10:00 min

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June 20, 2019

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, , ,
1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

Capítulos

  • 00:04Título
  • 00:57Preparation of Buffers, System, and Sample, and Loading of Sample
  • 03:42Preparation of the MALS Software and the FPLC Software
  • 05:49Analysis of SEC-MALS BSA Data
  • 08:04Results: SEC-MALS Analysis
  • 09:16Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了尺寸排除色谱与多角度光散射(SEC-MALS)的结合,用于对溶液中的蛋白质和复合物进行绝对表征。SEC-MALS 确定纯蛋白质、原生寡聚物、异质复合物和修饰性蛋白质(如糖蛋白)的分子量和大小。

Tags

Keywords: SEC-MALSSize-exclusion ChromatographyMulti-angle Light ScatteringMolecular WeightMacromoleculesOligomersComplexesGlycoproteinsMembrane ProteinsAbsolute MethodPeptidesPolymersProteinsNucleocapsidsPolysaccharidesVirus-like ParticlesVirusesPhosphate-buffered SalineFlow RateDRI Flow CellPurge ModeSystem CleanlinessRefractive Index

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