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Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
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JoVE Journal Pesquisa do Câncer
Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

DOI:
10.3791/59666-v

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06:09 min

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June 07, 2019

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, , , ,
1Department of Biomedical Sciences,Cornell University

Capítulos

  • 00:04Título
  • 00:35Skin Preparation and Ultraviolet (UV)-B Irradiation
  • 01:39Skin Isolation
  • 02:29Tissue Fixation
  • 03:06Frozen Tissue Block Preparation
  • 04:15Results: Representative Spatial and Temporal Control of Murine Melanoma Initiation
  • 05:50Conclusion

Summary

Tadução automática

Tadução automática

The following procedure describes a method for spatial and temporal control of melanocytic tumor initiation in murine dorsal skin, using a genetically engineered mouse model. This protocol describes macroscopic as well as microscopic cutaneous melanoma initiation.

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Keywords: Spatial ControlTemporal ControlMurine MelanomaMelanocyte Stem CellsMelanoma InitiationUV-B IrradiationSample Preparation

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