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Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
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Biologia
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JoVE Journal Biologia
Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

DOI:
10.3791/59673-v

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06:38 min

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August 14, 2019

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, , , , , , , , , ,
1Department of Nephrology,Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center,Shenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory,Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology,Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery,University of Alabama at Birmingham, 6People’s Hospital of Longhua

Capítulos

  • 00:04Título
  • 00:44Aorta Isolation
  • 02:21Porcine Aortic Endothelial Cell (pAEC) Isolation
  • 05:16Results: Representative Highly-Purified pAEC Identification
  • 05:53Conclusion

Summary

Tadução automática

Tadução automática

An effective enzymatic method for isolation of primary porcine aortic endothelial cells (pAECs) from miniature pigs is described. The isolated primary pAECs can be used to investigate the immune and coagulation response in xenotransplantation.

Tags

Primary Aortic Endothelial CellsMiniature PigsIsolationCultureEndotheliumXenotransplantationCollagenase IVAortaAbdominal IncisionHeparinCatheterHeartLungsCentrifugeWashing BufferDigestive SolutionIncubation

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