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Standardiseret og skalerbar analyse til undersøgelse af Perfuserende 3D Angiogen spiring af iPSC-afledte endotelceller in vitro
This content is Free Access.
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine,Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics,Leiden University, 3Ncardia, 4Department of Anatomy and Embryology,Leiden University Medical Center, 5Mimetas
Chapters
- 00:04Title
- 01:12Preparation of Device, Gel, and Coating
- 02:42Cell Seeding/Microvessel Culture
- 04:37Study Angiogenic Sprouting
- 05:49Study Anastomosis and Sprout Stabilization
- 06:50Fixation, Staining, and Imaging
- 08:55Results: Cell Seeding and 3D Sprouting of iPSC-EC
- 10:13Conclusion
Denne metode beskriver kulturen af IPSC-afledte endotelceller som 40 perfbrugte 3D mikrofartøjer i en standardiseret mikrofluidisk platform. Denne platform muliggør studiet af gradient-drevet angiogen spiring i 3D, herunder anastomose og stabilisering af de angiogene spirer i en skalerbar og høj gennemløb måde.
