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用于转化免疫肿瘤学(I-O)研究的人类外周血单核细胞(PBMC)的人工化异种移植模型

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A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research

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用于转化免疫肿瘤学(I-O)研究的人类外周血单核细胞(PBMC)的人工化异种移植模型

DOI:

10.3791/59679-v

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08:17 min

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August 15, 2019

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Zhuo Li*¹, Xiao Yang*¹, Yilu Zhang, Xiaolong Yang, Xinxin Cui, Yanjuan Zhang, Wenfeng Gong, Huichen Bai, Ning Liu, Zhiyu Tang, Mingming Guo, Kang Li, Tong Zhang, Lai Wang, Xiaomin Song

¹BeiGene (Beijing) Co., Ltd.

Capítulos

00:04Título
00:46Cyclophosphamide Myeloablation and Human Peripheral Blood Mononuclear Cell (PBMC) Transplantation and Tumor Engraftment
02:08Immunohistochemistry (IHC)
04:33In Vivo Efficacy and Pharmacodynamics Analyses
06:08Results: Representative PBMC- and Tumor-Engraftment and Anti-Tumor Antibody Treatment Analysis
07:16Conclusion

Summary

Tadução automática

我们描述了人类外周血单核细胞(PBMC)–基于人体的异种移植小鼠模型,用于转化免疫肿瘤学研究。该协议可作为建立和描述类似I-O治疗评估模型的一般准则。

Tags

Keywords: PBMC Humanized Xenograft Immuno-oncology Myeloid Cell Depletion Cyclophosphamide Disulfiram Tumor Growth Immunohistochemistry Antigen Retrieval

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