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小鼠视网膜冷冻节的制备,用于免疫性植物化学

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

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小鼠视网膜冷冻节的制备,用于免疫性植物化学

DOI:

10.3791/59683-v

•

05:25 min

•

July 01, 2019

•

Hélène Léger, Evelyn Santana, William A Beltran, Francis C Luca

¹Department of Biomedical Sciences,University of Pennsylvania School of Veterinary Medicine, ²Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine,University of Pennsylvania School of Veterinary Medicine

Capítulos

00:04Título
00:45Mouse Eye Enucleation, Eye Cup Dissection, and Embedding
03:08Sectioning Using a Cryostat
03:51Results: Immunohistochemistry of Mouse Retinal Cryo-sections
05:01Conclusion

Summary

Tadução automática

本报告介绍了制备冷冻小鼠视网膜部分用于免疫组织化学(IHC)的综合方法。所述方法包括眼部后杯的解剖、甲醛固定、嵌入最佳切割温度(OCT)介质和组织定向、切片和免疫染色。

Tags

Keywords: Mouse Retina Retinal Dissection Immunohistochemistry Cryo-section Eye Enucleation Eye Fixation Cornea Removal Lens Extraction Retina Isolation Cryo-protection Snap Freezing

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