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使用电压敏感染料在脑切片中进行宽场单光子光记录
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Neurociência
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JoVE Journal Neurociência
Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
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使用电压敏感染料在脑切片中进行宽场单光子光记录

DOI:
10.3791/59692-v

•

06:43 min

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June 20, 2019

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, , ,
1Laboratory for Neural Circuit Systems, Institute of Neuroscience,Tokushima Bunri University

Capítulos

  • 00:04Título
  • 00:54Brain Slice Preparation and Staining
  • 03:30Preparation of Experimental Apparatus
  • 04:27Recording Session
  • 05:22Results: Comparison of Optical Signal Between Mouse and Rat Hippocampal Slices
  • 06:24Conclusion

Summary

Tadução automática

Tadução automática

介绍了一种使用电压敏感染料的脑切片可重复、稳定的光学记录方法。本文介绍了电压敏感染料染色和记录光信号使用传统的海马切片制剂。

Tags

Keywords: Optical RecordingVoltage-sensitive DyeBrain SlicesWide-field Single-photonNeuropsychic DynamicsCircuit ChangesChemicalsBrain DevelopmentBrain ExtractionBrain SectioningVibratome SlicingBrain Slice RecoveryVoltage-sensitive Dye Staining

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