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新生大鼠脑组织在神经端子突形圆锥花序分布超微结构形态分析中的制备
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Neurociência
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JoVE Journal Neurociência
Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
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新生大鼠脑组织在神经端子突形圆锥花序分布超微结构形态分析中的制备

DOI:
10.3791/59694-v

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10:09 min

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June 07, 2019

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,
1Department of Anesthesiology,University of Virginia Health System, 2Department of Anesthesiology,Universita’ degli Studi di Padova

Capítulos

  • 00:04Título
  • 01:27Post-fixation, Sequential Dehydration, and Staining of Brain Sections
  • 03:38Infiltration and Embedding
  • 05:01Flat Embedding
  • 06:09Capsule Embedding
  • 07:28Results: Electron Micrographs of Newborn Rat Brain Tissue
  • 09:02Conclusion

Summary

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Tadução automática

我们描述了处理新生大鼠脑组织以获得高分辨率的电子显微镜的程序, 以进行神经末端突触囊分布的形态分析。用这些方法获得的显微图像也可用于研究许多其他细胞成分的形态及其尺寸结构关系。

Tags

Newborn Rat BrainUltrastructural Morphometric AnalysisSynaptic Vesicle DistributionNerve TerminalsTransmission Electron MicroscopyOsmium TetroxidePhosphate BufferUranyl AcetateSynaptic FunctionSynaptic DevelopmentAnesthetics

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