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rRNA 2'-O-甲基化的脱氧核糖核酸依赖性分析
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
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rRNA 2'-O-甲基化的脱氧核糖核酸依赖性分析

DOI:
10.3791/59700-v

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09:12 min

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September 16, 2019

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,
1School of Biosciences,University of Birmingham

Capítulos

  • 00:04Título
  • 00:29DNAzyme Design
  • 01:46 S. cerevisiae Growth and RNA Isolation
  • 02:22DNAzyme Digestion
  • 05:07RNA Purification and Electrophoresis
  • 07:27Results: Analysis of Site-specific Methylation with 10-23 and 8-17 DNAzymes
  • 08:21Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了RNADNA依赖性裂解的协议。这使得RNA2'-O-甲基化能够进行快速和位点依赖性分析。这种方法可用于对打用RNA活性的初步或主要评估。

Tags

Keywords: DNAzymeRNA MethylationSnoRNAYeastBiologia MolecularRNA IsolationRNA AnalysisRNA Digestion10-23 DNAzyme8-17 DNAzymeSaccharomyces Cerevisiae

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