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将鼠皮皮和皮米与热解辛分离，以检测部位特异性炎症mRNA和蛋白质

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

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将鼠皮皮和皮米与热解辛分离，以检测部位特异性炎症mRNA和蛋白质

DOI:

10.3791/59708-v

•

08:45 min

•

September 29, 2021

•

Vikramsingh Gujar*¹, Michael B. Anderson*¹, Kenneth E. Miller*¹, Radhika D. Pande, Pranav Nawani, Subhas Das

¹Department of Anatomy and Cell Biology,Oklahoma State University Center for Health Sciences, ²Institute for Shock Physics,Washington State University

Capítulos

00:04Introduction
01:21Carrageenan-Induced Inflammation (C-II)
02:31Thermolysin Epidermis and Dermis Separation
04:11Tinctorial Histology
05:43Results: Representative Site-Specific Inflammatory mRNA and Protein Detection
07:40Conclusion

Summary

Tadução automática

这里介绍的是一个表皮与真皮分离的协议，用于评估炎症调解器的产生。炎症后，大鼠后爪表皮在4°C下由热利辛从真皮中分离出来。然后，通过RT-PCR进行表皮分析，通过西方污点和免疫造血术进行蛋白质评估。

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