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Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

DOI:
10.3791/59709-v

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06:25 min

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July 10, 2019

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, , , , , ,
1Wildlife Zoonoses & Vector-Borne Diseases Research Group,Animal and Plant Health Agency, 2Institute of Infection and Global Health,University of Liverpool

Capítulos

  • 00:04Título
  • 00:58Preparation of RNA Dilution Series
  • 01:22Real-time PCR
  • 03:33Data Analysis
  • 04:28Results: Pan-lyssavirus RT-PCR
  • 05:38Conclusion

Summary

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Tadução automática

This real-time RT-PCR using dsDNA intercalating dye is suitable to diagnose lyssavirus infections. The method begins with RNA extracted from rabies suspected ante-mortem or post-mortem samples, detailing master mix preparation, RNA addition, setup of the real-time machine and correct interpretation of results.

Tags

Keywords: Pan-LyssavirusReal Time RT-PCRRabies DiagnosisLyssavirus GenusMolecular AssaysRNA QuantificationPCR Master MixBeta-ActinSYBR GreenDissociation Curve

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