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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
JoVE Journal
Pesquisa do Câncer
This content is Free Access.
JoVE Journal Pesquisa do Câncer
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
DOI:

06:53 min

June 08, 2019

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Capítulos

  • 00:04Título
  • 00:59Target DNA Detection and Quantification
  • 03:19Data Analysis
  • 05:00Results: Representative Mutation Analyses in Pre-amplified Plasma and Cerebrospinal Fluid (CSF) Cell Free DNA (cfDNA)
  • 06:32Conclusion

Summary

Tadução automática

Here, we present a protocol to detect tumor somatic mutations in circulating DNA present in patient biological fluids (biofluids). Our droplet digital polymerase chain reaction (dPCR)-based method enables quantification of the tumor mutation allelic frequency (MAF), facilitating a minimally invasive complement to diagnosis and temporal monitoring of tumor response.

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