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An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
DOI:

07:55 min

July 06, 2019

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Capítulos

  • 00:04Título
  • 00:37Setting up Crystallization Drops and Observing Crystal Growth
  • 01:56Crystal Manipulation
  • 04:23In Situ Diffraction Data Collection at Ambient Temperature and at Cryogenic Temperature
  • 06:20Results: Novel Sample Holder in Macromolecular X-Ray Crystallography for Crystal Growth, Crystal Manipulation, and X-Ray Diffraction Data Collection
  • 07:23Conclusion

Summary

Tadução automática

A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.

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