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DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
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Capítulos
- 00:04Título
- 01:29Primase DNA Binding Experiment: Blocking Procedure
- 02:44Primase DNA Binding Experiment: Protein Binding
- 03:22Primase DNA Binding Experiment: Fluorescent Antibody Attachment
- 05:15Results: Analysis of High-Throughput Primase Profiling
- 06:42Conclusion
Protein binding microarray (PBM) experiments combined with biochemical assays link the binding and catalytic properties of DNA primase, an enzyme that synthesizes RNA primers on template DNA. This method, designated as high-throughput primase profiling (HTPP), can be used to reveal DNA-binding patterns of a variety of enzymes.
