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用心脏灌注和二固定仪对脑和垂体血管进行标记

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative

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用心脏灌注和二固定仪对脑和垂体血管进行标记

DOI:

10.3791/59768-v

•

05:53 min

•

June 13, 2019

•

Romain Fontaine, Finn-Arne Weltzien

¹Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine,Norwegian University of Life Sciences

Capítulos

00:04Título
00:44Instrument Preparation
01:33Medaka Dissection and Perfusion
03:56Results: Representative Brain and Pituitary Imaging After Cardiac Perfusion with a Dil-fixative
05:05Conclusion

Summary

Tadução automática

本文介绍了一种快速的协议, 通过心脏灌注的心脏灌注的 DiI 稀释在固定, 使用 medaka (Oryzias latipes) 作为模型, 并侧重于大脑和垂体组织的远端鱼的血管标记。

Tags

Blood Vessels Teleost Brain Pituitary Cardiac Perfusion DiI-fixative Medaka Vascular Anatomy Neuron Endocrine Cell Polystyrene Fish Holder Perfusion System DiI-fixative Solution Euthanized Fish Ventricle Bulbus Arteriosus Sinus Venosus

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