Comparative Lesions Analysis Through a Targeted Sequencing Approach

Caterina Vicentini; Andrea Mafficini; Michele Simbolo; Matteo Fassan; Pietro Delfino; Rita  Teresa Lawlor; Borislav Rusev; Aldo Scarpa; Vincenzo Corbo

doi:10.3791/59844

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Comparative Lesions Analysis Through a Targeted Sequencing Approach

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Comparative Lesions Analysis Through a Targeted Sequencing Approach

DOI:

10.3791/59844-v

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08:16 min

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November 05, 2019

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Caterina Vicentini, Andrea Mafficini, Michele Simbolo, Matteo Fassan, Pietro Delfino, Rita Teresa Lawlor, Borislav Rusev, Aldo Scarpa², Vincenzo Corbo²

¹ARC-Net Research Centre,University and Hospital Trust of Verona, ²Department of Diagnostics and Public Health, Section of Pathology,University and Hospital Trust of Verona, ³Department of Medicine (DIMED), Surgical Pathology and Cytopathology Unit,University of Padua

Capítulos

00:04Título
00:48Library Pooling and Sequencing Run
02:28Sample Library Dilution and Loading
03:00Next Generation Sequencing
03:43Mutation and Copy-Number Variation (CNV) Analysis
05:00Somatic Mutation Indexing
05:36Results: Representative Comparative Lesion Analyses
07:16Conclusion

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This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.

Comparative Lesions Analysis Through a Targeted Sequencing Approach

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