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In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
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In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

DOI:
10.3791/59849-v

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11:07 min

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August 14, 2019

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, ,
1Department of Molecular Medicine and Pathology, School of Medical Sciences,University of Auckland

Capítulos

  • 00:06Título
  • 00:44Preparation of Embryos for Implantation
  • 01:31Perivitelline Injection of Mammalian Cancer Cells into 2 dpf (Day Post Fertilization) Embryos
  • 03:55Live Imaging and Quantitation of the Angiogenic Response to the Zebrafish Xenograft
  • 08:34Results: Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
  • 10:37Conclusion

Summary

Tadução automática

Tadução automática

The aim of this method is to generate an in vivo model of tumor angiogenesis by xenografting mammalian tumor cells into a zebrafish embryo that has fluorescently-labelled blood vessels. By imaging the xenograft and associated vessels, a quantitative measurement of the angiogenic response can be obtained.

Tags

Keywords: In Vivo ImagingTumor XenograftsZebrafishAngiogenesisVascularizationQuantitationB16-F1 CellsMicroinjectionTransgenic EmbryosECMYolk Sac

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