Journal
/
Pesquisa do Câncer
/
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
JoVE Journal
Pesquisa do Câncer
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Pesquisa do Câncer
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

DOI:
10.3791/59895-v

•

09:49 min

•

July 05, 2019

•

, ,
1Department of Pathology,University of Colorado – Anschutz Medical Campus

Capítulos

  • 00:04Título
  • 00:49Random Priming and First Strand cDNA Synthesis
  • 01:55Ligation Step 2 and Bead Purification
  • 04:25First PCR and Bead Purification
  • 05:48Library Quantitation and Sequencing
  • 07:41Assay Data Interpretation
  • 08:39Results: Sample Summary and Fusion Calls
  • 09:27Conclusion

Summary

Tadução automática

Tadução automática

This article details the use of an anchored multiplex polymerase chain reaction-based library preparation kit followed by next-generation sequencing to assess for oncogenic gene fusions in clinical solid tumor samples. Both wet-bench and data analysis steps are described.

Tags

Keywords: Gene Fusion DetectionAnchored Multiplex PCRNext-generation SequencingOncogenic Gene FusionsTumor SamplesFormulative FixationDiagnosisPrognosisTreatment SelectionArtifactsRandom PrimingFirst Strand CDNA SynthesisSecond Strand CDNA SynthesisBead PurificationMolecular Barcode Adapter

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/59895?article_type=v

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
check_url/pt/59895?article_type=v

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
check_url/pt/59895?article_type=v

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
check_url/pt/59895?article_type=v

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
check_url/pt/59895?article_type=v

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
check_url/pt/59895?article_type=v

Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
check_url/pt/59895?article_type=v

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
check_url/pt/59895?article_type=v

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
check_url/pt/59895?article_type=v

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
check_url/pt/59895?article_type=v

Transmitochondrial Cybrid Generation Using Cancer Cell Lines (Video) | JoVE

Read Article