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使用锚定多路聚合酶链反应的致癌基因融合检测,随后进行下一代测序
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Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
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使用锚定多路聚合酶链反应的致癌基因融合检测,随后进行下一代测序

DOI:
10.3791/59895-v

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09:49 min

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July 05, 2019

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, ,
1Department of Pathology,University of Colorado – Anschutz Medical Campus

Capítulos

  • 00:04Título
  • 00:49Random Priming and First Strand cDNA Synthesis
  • 01:55Ligation Step 2 and Bead Purification
  • 04:25First PCR and Bead Purification
  • 05:48Library Quantitation and Sequencing
  • 07:41Assay Data Interpretation
  • 08:39Results: Sample Summary and Fusion Calls
  • 09:27Conclusion

Summary

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Tadução automática

本文详细介绍了使用锚定多路聚合酶链式反应基库制备试剂盒,然后进行下一代测序,以评估临床固体肿瘤样本中的致癌基因融合。介绍了湿凳和数据分析步骤。

Tags

Gene Fusion DetectionAnchored Multiplex PCRNext-generation SequencingOncogenic Gene FusionsTumor SamplesFormulative FixationDiagnosisPrognosisTreatment SelectionArtifactsRandom PrimingFirst Strand CDNA SynthesisSecond Strand CDNA SynthesisBead PurificationMolecular Barcode Adapter

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