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Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
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Genética
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JoVE Journal Genética
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

DOI:
10.3791/59897-v

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09:21 min

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September 13, 2019

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,
1Graduate School of Biomedical Sciences,Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery,University California San Diego

Capítulos

  • 00:04Título
  • 01:44Cell Isolation and Culture
  • 03:46Tumorsphere Derivation
  • 05:02Tumorsphere Preparation for Allograft Transplantation
  • 07:26Results: Tumorsphere Derivation and Transfection
  • 08:46Conclusion

Summary

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This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.

Tags

RhabdomyosarcomaTumorsphere Formation AssayCell Of OriginMyogenic MarkersTumor-originating CellsSpheroid StructureDrug-screeningCell IsolationCollagenase DigestionCell Suspension

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