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使用点击化学测量血管平滑肌细胞增殖
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Biologia
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JoVE Journal Biologia
Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry
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使用点击化学测量血管平滑肌细胞增殖

DOI:
10.3791/59930-v

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07:17 min

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October 30, 2019

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, ,
1Department of Neuroscience, Cell Biology, and Physiology,Wright State University, 2Department of Surgery, Boonshoft School of Medicine,Wright State University

Capítulos

  • 00:05Título
  • 00:30Vascular Smooth Muscle Cell Labelling with EdU and Fixing with PFA
  • 01:53Detection of EdU Using Fluorescence
  • 02:50Detection of EdU Using Luminescence
  • 04:40Results: Proliferation of Vascular Smooth Muscle Cells Measurements
  • 06:06Conclusion

Summary

Tadução automática

Tadução automática

增殖是细胞功能的关键部分，也是用于评估新药潜在毒性的常见读物。因此，测量增殖是细胞生物学中常用的测定方法。在这里，我们提出了一种简单、通用的测量增殖方法，可用于附着细胞和非粘附细胞。

Tags

Vascular Smooth Muscle CellsCell ProliferationClick ChemistryEdUFluorescenceLuminescenceCell AssayPDGFParaformaldehydeTX-100THPTACopper SulfateFluorescein Picolyl-azideSodium AscorbateDAPIHRP AzideELISA Substrate

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