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通过全基因组互惠性分析,对糖母菌菌种间热耐性差异的遗传图谱

JoVE Journal
Genética

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JoVE Journal Genética

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

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通过全基因组互惠性分析,对糖母菌菌种间热耐性差异的遗传图谱

DOI:

10.3791/59972-v

•

10:08 min

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August 12, 2019

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Carly V. Weiss², Julie N. Chuong, Rachel B. Brem³

¹Department of Plant and Microbial Biology,University of California Berkeley, ²Department of Biology,Stanford University, ³Buck Institute for Research on Aging

Capítulos

00:04Título
00:22Preparation of Hybrid Yeast Cells for Transformation
01:55Transformation of pJR487 into Hybrid Yeast Cells
08:16Results: Thermotolerance Differences between Species of Saccharomyces
09:44Conclusion

Summary

Tadução automática

通过测序(RH-seq)的互惠血性是绘制物种间特征差异遗传基础的有力新方法。血吸血池由转位子诱变产生,其适应性通过高全测序的竞争性增长进行跟踪。对结果数据的分析可确定特征背后的基因。

Tags

Genetic Mapping Thermotolerance Saccharomyces Yeast Genome-wide Reciprocal Hemizygosity Analysis High-throughput High Resolution Freezer Stock YPD Agar Liquid YPD Optical Density Back Dilution Tris-EDTA Buffer Lithium Acetate Polyethylene Glycol

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