Journal
/
Pesquisa do Câncer
/
利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞
JoVE Journal
Pesquisa do Câncer
This content is Free Access.
JoVE Journal Pesquisa do Câncer
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
Please note that all translations are automatically generated. Click here for the English version.

利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞

DOI:
10.3791/59997-v

•

10:57 min

•

October 24, 2019

•

, , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Immunology, Institute for Frontier Life and Medical Sciences,Kyoto University

Capítulos

  • 00:04Título
  • 00:58Passaging Human iPSCs on Mouse Embryonic Fibroblasts
  • 02:30Preparation of OP9/DLL1 Cells for Co-culture with hiPSCs
  • 03:51In Vitro Differentiation of hiPSCs into CD8αβ+
  • 08:38Results: hiPSC-derived Hematopoietic Progenitor Cells
  • 09:55Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了一种利用OP9/DLL1共培养系统生成功能性肿瘤抗原特异性诱导多能干细胞衍生CD8++单阳性T细胞的方法。

Tags

Keywords: Human Induced Pluripotent Stem CellsTumor Antigen-specific T CellsOP9-Delta-1 Co-cultureT Cell GenerationRegenerative MedicineCell-based TherapyNK CellsCell DifferentiationCell PassagingMouse Embryonic FibroblastsOP9 Media

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/59997?article_type=v

应用 Fraumeni 综合征诱导多潜能干细胞建模骨肉瘤
check_url/pt/59997?article_type=v

生成毛因诱导多能干细胞衍生的肿瘤抗原特异性胸腺移民的三维胸腺培养系统
check_url/pt/59997?article_type=v

cd8+ t 细胞诱导体外肿瘤细胞凋亡的实时检测--研究肿瘤浸润髓内细胞的免疫抑制作用
check_url/pt/59997?article_type=v

制造用于采用免疫疗法的奇美抗原受体（CAR）T细胞
check_url/pt/59997?article_type=v

细胞毒性细胞因子诱导的杀伤性T细胞的分离和扩张，用于癌症治疗
check_url/pt/59997?article_type=v

正交胰腺肿瘤的产生与肿瘤渗透T细胞细胞毒性的外体特征
check_url/pt/59997?article_type=v

靶性抗原特异性治疗抗体的肿瘤及组织分布分析
check_url/pt/59997?article_type=v

肿瘤细胞外侧尾静脉注射后肺转移的病理分析
check_url/pt/59997?article_type=v

肿瘤移植用于评估小鼠肿瘤浸润CD8 + T细胞的动力学
check_url/pt/59997?article_type=v

体内 通过脾T细胞流式细胞术筛选肿瘤来源的细胞外囊泡的免疫原性

Read Article