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Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
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Biologia
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JoVE Journal Biologia
Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

DOI:
10.3791/60003-v

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07:53 min

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June 04, 2020

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, ,
1Imaging Facility,Max Planck Institute of Immunobiology and Epigenetics

Capítulos

  • 00:05Introduction
  • 00:47Live Cell Labeling
  • 01:35Superresolution Structured Illumination Microscopy (SR-SIM) Image Acquisition
  • 03:54Data Analysis
  • 05:18Results: Representative Live Cell Labeling and Mitochondrial Nucleoid Tracking
  • 07:01Conclusion

Summary

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The protocol describes specific labeling of mitochondrial nucleoids with a commercially available DNA gel stain, acquisition of time lapse series of live labeled cells by super-resolution structured illumination microscopy (SR-SIM), and automatic tracking of nucleoid motion.

Tags

Mitochondrial NucleoidsTime-lapseStructured Illumination MicroscopySYBR GoldHeLa CellsLive Cell ImagingSuper-resolution MicroscopyElectron Multiplying CCD Camera

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