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An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

DOI:
10.3791/60031-v

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08:02 min

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October 19, 2019

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, , ,
1School of Life Sciences,The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences, Shenzhen

Capítulos

  • 00:04Título
  • 00:56Coverslip Preparation
  • 01:36Rat Embryonic Neuron Dissection
  • 02:51Primary Cortical Neuron Culture
  • 04:08Cell Transfection and Fixation
  • 05:16Neurite Overgrowth Measurement
  • 05:58Results: Representative Neurite Growth Modulation
  • 07:29Conclusion

Summary

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In this report, we describe a simple protocol for studying neurite outgrowth in embryonic rat cortical neurons by co-transfecting with EGFP and the protein of interest.

Tags

EGFPNeurite OutgrowthPrimary NeuronsTransfectionNeural RegenerationBrain TraumaNeurodegenerative DiseasesPoly-D-LysineSprague Dawley RatCerebral HemispheresCortexTrypsin EDTAMaintenance Medium

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