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Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption
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Bioquímica
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JoVE Journal Bioquímica
Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

DOI:
10.3791/60034-v

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09:20 min

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October 04, 2019

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,
1Department of Mechanical and Aerospace Engineering,University of Central Florida, 2Burnett School of Biomedical Sciences, College of Medicine,University of Central Florida

Capítulos

  • 00:04Título
  • 01:22Making Polyacrylamide (PA) Gels
  • 03:15Micropattern Stencil Preparation
  • 04:23Collagen-I Hydrogel Coating
  • 05:26Creating HUVEC Monolayers on Hydrogels
  • 06:57Results: The Impact of Chalcone Treatment
  • 08:14Conclusion

Summary

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Here, we present a mechanics-based protocol to disrupt the gap junction connexin 43 and measure the subsequent impact this has on endothelial biomechanics via observation of tractions and intercellular stresses.

Tags

Keywords: Endothelial BiomechanicsConnexin 43Cell-cell JunctionsCell-generated Mechanical ForcesTractionsIntercellular StressorsOrgan-on-chipIn Vitro Drug DeliveryBind-silane SolutionHydrogelFluorescent BeadsAcrylamideBisacrylamideAmmonium PersulphateTEMED

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